家蚕肠道微生物多样性及功能研究进展
唐名艳;蒋满贵;唐亮;赵烨芸;黄深惠;陈小青;
摘要(Abstract):
为了探索家蚕肠道微生物与家蚕健康生长之间的关系,增强家蚕抗病能力、提高桑叶转化率、提高蚕茧的产量和质量,基于国内外研究进展,系统地论述了家蚕肠道微生物的多样性及其影响因素,阐述了肠道微生物在家蚕体内发挥的作用,特别是在防御和解毒方面的功能。就目前家蚕肠道微生物研究存在的问题进行阐述,提出今后应建立更加丰富的家蚕肠道微生物多样性数据库,分离培养获得更多家蚕肠道微生物用于功能验证,阐明肠道菌在维护家蚕健康和抗病方面的机制。建议加大开发蚕用微生态制剂,识别家蚕肠道内与杀虫剂抗性相关的细菌种类的研究方向,以期为推动家蚕肠道功能微生物开发和应用提供参考。
关键词(KeyWords): 家蚕;肠道微生物;多样性;研究进展
基金项目(Foundation): 国家现代农业产业技术体系建设专项(CARS-18);; 广西科技重大专项(桂科AA19182012-4)
作者(Authors): 唐名艳;蒋满贵;唐亮;赵烨芸;黄深惠;陈小青;
DOI: 10.19553/j.cnki.1006-1657.2022.01.008
参考文献(References):
- [1] LEULIER F,MAC NEIL L T,LEE W J,et al.Integrative physiology:at the crossroads of nutrition,microbiota,animal physiology,and human health[J].Cell Metabolism,2017,25(3):522-534.
- [2] SHARON G,SEGAL D,RINGO JM,et al. Commensal bacteria play a role in mating preference of drosophila melanogaster[J]. Proceedings of the National Academy of Sciences of the United States of America,2010(107):20051-20056.
- [3] WARNECKE F,LUGINBUEHL P,IVANOVA N,et al.Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite[J]. Nature,2007(450):560-565.
- [4] OLIVER K,MORAN N,HUNTER M. Variation in resistance to parasitism in aphids is due to symbionts not host genotype[J]. Proceedings of the National Academy of Sciences,2005,102(36):12795-12800.
- [5] DILLON R J,VENNARD C T,CHARNLEY A K. A note:gut bacteria produce components of a locust cohesion pheromone[J]. J Appl Microbiol,2009,49(2):114-119.
- [6]汤历,廉婕,陆小军,等.德国小蠊肠道细菌抗真菌的初步研究[J].昆虫天敌,2005(3):140-144.
- [7] DUNBAR H E,WILSON A C C,FERGUSON N R,et al.Aphid thermal tolerance is governed by a point mutation in bacterial symbionts[J]. PLoS Biol,2007,5(5):1006-1015.
- [8] BRODERICK N A,RAFFA K F,HANDELSMAN J.Midgut bacteria required for Bacillus thuringiensis insecticidal activity[J]. Proceedings of the National Academy of Sciences,2006,103(41):15196-15199.
- [9]TAKIZAWA Y,IIZUKA T. The aerobic bacterial flora in the gut of larvae of the silkworm,Bombyx mori L[J].The Journal of Sericultural Science of Japan,1968,37(4):295-305.
- [10]MENIZIBEYA O W. Gastrointestinal Physiology[M].Springer,Cham,2018.
- [11]SCHLEIFER K H,KILPPERBALZ R. Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov[J].International Journal of Systematic Bacteriology,1984,34(1):31-34.
- [12]鲁兴萌,金伟.桑蚕细菌性肠道病研究[J].蚕桑通报,1996(2):1-3.
- [13]ISHII K,ADACHI T,HARA T,et al. Identification of a Serratia marcescens virulence factor that promotes hemolymph bleeding in the silkworm,Bombyx mori[J]. Journal of Invertebrate Pathology,2014,117:61-67.
- [14]TAO H P,SHEN Z Y,ZHU F,et al. Isolation and identification of a pathogen of silkworm Bombyx mori[J]. Current Microbiology,2011,62(3):876-883.
- [15]陈勃生.家蚕肠道微生物的多样性及其代谢功能探究[D].杭州:浙江大学,2020.
- [16]相辉,李木旺,赵勇,等.家蚕幼虫中肠细菌群落多样性的PCR-DGGE和16S rDNA文库序列分析[J].昆虫学报,2007(3):222-233.
- [17]董慧玲.不同饲料对家蚕血淋巴代谢组学及肠道微生物多样性的影响[D].苏州:苏州大学,2017.
- [18]郝长富,李刚,孙熙,等.不同饲料饲育的家蚕幼虫肠道细菌的多样性分析[J].昆虫学报,2019,62(1):61-72.
- [19]向芸庆.不同饲料饲养家蚕其肠道微生态优势菌群的类型组成及差异性研究[D].重庆:西南大学,2010.
- [20]李莉.桑叶表面沙尘对家蚕肠道菌群及消化的影响[D].阿拉尔:塔里木大学,2021.
- [21]张剑飞,王林玲,周泽扬.家蚕K100E23肠道微生物初探[J].四川蚕业,2002(3):8-12.
- [22]许刚,孙振丽,胡小龙,等.基于16S r RNA基因序列分析家蚕肠道细菌的多样性[J].蚕业科学,2015,41(4):641-649.
- [23] KUMAR D,SUN Z,CAO G,et al. Bombyx mori bidensovirus infection alters the intestinal microflora of fifth instar silkworm(Bombyx mori)larvae[J]. Journal of Invertebrate Pathology,2019,163:48-63.
- [24]KUMAR D,SUN Z,CAO G,et al. Study of gut bacterial diversity of Bombyx mandarina and Bombyx mori through 16S r RNA gene sequencing[J]. Journal of Asia-Pacific Entomology,2019,22(2):522-530.
- [25]LI F C,LI M X,MAO T T,et al. Effects of phoxim exposure on gut microbial composition in the silkworm,Bombyx mori[J]. Ecotoxicology and Environmental SafetyVolume,2020(189):110011.
- [26]李刚,吴柯成,郝长富,等.基于16S rDNA序列对蓖麻蚕肠道细菌多样性的研究[J].蚕业科学,2018,44(6):867-874.
- [27]袁志辉,蓝希钳,杨廷,等.家蚕肠道细菌群体调查与分析[J].微生物学报,2006,46(2):285-291.
- [28]田贞华,惠丰立,柯涛,等.家蚕肠道细菌种群结构分析[J].蚕业科学,2007(4):592-595.
- [29]孙雪奇,黄玉祥,董长江,等.家蚕肠道好氧和部份兼厌氧微生物及蚕用微生态制剂的研究[J].四川蚕业,1996(1):13-15.
- [30]李蒙英,许宏庆,虞晓华,等.家蚕肠道菌群的研究[J].江苏蚕业,2000(2):5-7.
- [31]陈林.纳米银对家蚕生长发育及肠道细菌的影响[D].重庆:西南大学,2020.
- [32]岳玲.家蚕杂交种F1及其亲本的肠道微生物多样性和血淋巴代谢组学差异研究[D].苏州:苏州大学,2018.
- [33]卢延.葡萄球菌在家蚕肠道中的种群分布、系统发育及相关病理和生理作用的研究[D].重庆:西南大学,2020.
- [34]费仁雷,朱幼玲,夏克详,等.几种实验昆虫的微生物分离培养及初步鉴定[C]//中南地区实验动物科技交流会.南宁,2009.
- [35]陈蕾,周小理,周一鸣,等.高通量测序技术在肠道菌群研究中的应用[J].食品工业,2016,37(3):269-273.
- [36]韩益飞,徐世清,陈息林,等.现行主要蚕品种高温适应性的初步试验[J].中国蚕业,2003(3):46-48.
- [37]孙振丽.病毒感染和高温处理对家蚕肠道菌群结构的影响[D].苏州:苏州大学,2016.
- [38]杜贝贝.高温对家蚕肠道微生物组成及功能的影响[D].苏州:苏州大学,2017.
- [39]李冠楠.氟胁迫对不同抗性家蚕肠道微生态环境的影响[D].重庆:西南大学,2015.
- [40]GU Z Y,LI F C,HU J S,et al. Sublethal dose of phoxim and Bombyx mori nucleopolyhedrovirus interact to elevate silkworm mortality[J]. Pest Management Science,2017,73(3):554-561.
- [41]高绘菊,路国兵,查传勇,等.家蚕肠道产酶菌的分离与筛选[J].蚕业科学,2007,33(2):228-233.
- [42]PREM ANAND A A,VENNISON S J,SANKAR S G,et al. Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose,xylan,pectin and starch and their impact on digestion[J].Journal of Insect Science,2010,10(1):107.
- [43]王在贵,杨文静,刘朝良,等.家蚕肠道产蛋白酶菌株的分离与鉴定及其发酵条件[J].农业生物技术学报,2011,19(01):149-156.
- [44]杨文静,王在贵,刘朝良,等.家蚕肠道产淀粉酶细菌的分离鉴定及其酶基因的克隆与表达[J].激光生物学报,2011,20(2):219-229.
- [45]冯伟.家蚕肠道微生态产脂肪酶细菌的分离鉴定与差异性比较[D].重庆:西南大学,2011.
- [46]陈睿,王在贵,杨文静,等.家蚕肠道产蛋白酶细菌的分离筛选与分子鉴定[J].中国微生态学杂志,2011,23(8):681-683,688.
- [47]马如箭.家蚕肠道纤维素酶菌株的筛选及生化特性的研究[D].镇江:江苏科技大学,2013.
- [48]R. RAJAGOPAL. Beneficial interactions between insects and gut bacteria[J]. Indian Journal of Microbiology,2009,49(2):114-119.
- [49]鲁兴萌,汪方炜.家蚕肠球菌对微孢子虫体外发芽的抑制作用[J].蚕业科学,2002(2):126-128.
- [50]汪方炜,鲁兴萌,黄少康.家蚕肠球菌体外抑制微孢子发芽的动力学研究[J].蚕业科学,2003(2):157-161.
- [51]宋连花.家蚕来源肠球菌的RAPD分型及类肠球菌素的分离纯化[D].泰安:山东农业大学,2006.
- [52] WEI F,WANG X Q,WEI Z,et al. Isolation and Characterization of Lipase-Producing Bacteria in the Intestine of the Silkworm,Bombyx mori,Reared on Different Forage[J]. Journal of Insect Science,2011,11(135):1-10.
- [53]王晓强.不同环境因子及微生物菌株对家蚕抗性生理影响的初步研究[D].重庆:西南大学,2011.
- [54]LIU R H,WANG W H,LIU X Y,et al. Characterization of a lipase from the silkworm intestinal bacterium bacillus pumilus with antiviral activity against Bombyx mori(Lepidoptera:Bombycidae)nucleopolyhedrovirus In vitro.[J]. Journal of insect science,2018,18(6):3.
- [55]RHEINALLT M J,CHIRAYU D,TREVOR M D,et al.Lactobacilli modulate epithelial cytoprotection through the Nrf2 Pathway[J]. Cell Reports,2015,12(8):1217-1225.
- [56]BERASATEGUI A,AXELSSON K,NORDLANDER G,et al. The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles[J]. Molecular Ecology,2016,25(16):4014-4031.
- [57] CHENG D F,GUO Z J,RIEGLER M,et al. Gut symbiont enhances insecticide resistance in a significant pest,the oriental fruit fly Bactrocera dorsalis(Hendel)[J]. Microbiome,2017,5(1):13.
- [58]PANG R,CHEN M,YUE L,et al. A distinct strain of Arsenophonus symbiont decreases insecticide resistance in its insect host[J]. PLOS Genetics,2018,14(10):e1007725.
- [59] LIU X D,GUO H F. Importance of endosymbionts Wolbachia and Rickettsia in insect resistance development.[J]. Current opinion in insec science,2019(33):84-90.
- [60]CHEN B S,ZHANG N,XIE S,et al. Gut bacteria of the silkworm Bombyx mori facilitate host resistance against the toxic effects of organophosphate insecticides[J].Environment International,2020,10(143):105886.
- [61]吴民. EM技术应用于蚕业生产的试验初报[J].畜禽业,1998(10):13-14.
- [62]匡英秋,杜贤明,宋铭件,等. Em原露在家蚕饲育中的应用试验初报[J].北方蚕业,2000(2):19-20.
- [63]磨长寅. EM应用于蚕业生产的试验初报[J].广西蚕业,2000,37(2):6-7.
- [64]杭柏林,胡建和,赵万勇,等. EM在种桑养蚕上的应用[J].广东蚕业,2008,42(2):44-46.
- [65]易发平.家蚕肠道好氧微生物菌群分析及微生态环境调控研究[D].重庆:西南农业大学,2001.
- [66]张云骅,李建洪,万虎.昆虫共生菌与宿主的解毒代谢关系研究进展[J].农药学学报,2019,21(5-6):729-735.
文章评论(Comment):
|
||||||||||||||||||
|