陆春霞;董桂清;梁贵秋;潘志新;周晓玲;刘开莉;肖潇;黄正勇;吴婧婧;

• 1:广西壮族自治区蚕业技术推广总站

摘要(Abstract)：

桑黄是珍贵的食用药真菌,含有多糖、黄酮、多酚、萜类等活性成分,为了探索桑黄在药用领域的开发价值以及人工栽培技术的发展状况,就国内外研究学者在桑黄的活性成分和作用机理、人工栽培方面的研究以及存在的问题和展望进行综述,旨在为我国今后在桑黄资源产业化开发方面提供参考的依据。

关键词(KeyWords)： 桑黄;活性成分;药理作用;人工栽培

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 陆春霞;董桂清;梁贵秋;潘志新;周晓玲;刘开莉;肖潇;黄正勇;吴婧婧;

DOI: 10.19553/j.cnki.1006-1657.2019.04.009

参考文献(References)：

• [1]张小青，戴玉成.中国真菌志[M].北京：科学出版社，2005:117-191.
• [2]戴玉成，崔宝凯.药用真菌桑黄种类研究[J].北京林业大学学报，2014,36(5):1‐6.
• [2]王超儀，包海鹰.6种“桑黄”石油醚提取物的体内抗肿瘤活性[J].菌物研究，2013,11(3):196-201．
• [4]JEON T I,JUNG C H,CHO J Y,et al.Identification of an an-ticancer compound against HT-29 cells from phellinuslinteus grown on germinated brown rice[J].Asian Pac J Trop Biomed,2013,3(10):785-789.
• [5]PARK H J,HAN E S,PARK D K,et al.An extract of phelli-nus linteus grown on germinated brown rice inhibits in-flammation markers in RAW264.7 macrophages by sup-pressing inflammatory cytokines,chemokines,and media-tors and up-regulating antioxidant activity[J].JMed Food,2010,13(6):1468-1477.
• [6]NOH J R,LEE I K,KIM Y H,et al.Antiatherogenic effect of antioxidant polyphenols from phellinus baumii in apoli-poprotein E-deficient mice[J].Ann Nutr Metab,2011,2(59):145-153.
• [7]DAI Y C,ZHOU L W,CUI B K,et al.Current advances in Phellinus sensu lato:medicinal species,functions,metabolites and mechanisms[J].Appl Microbiol Biotechnol,2010,87(5):1587.
• [8]张维博，王家国，李正阔，等.药用真菌桑黄的研究进展[J].中国中药杂志，2014,39(15):2838-2845.
• [9]闫国卿.桑黄和鼠妇化学成分研究[D].合肥：安徽大学，2014.
• [10]王清，沈业寿，赵浩如.桑黄子实体水提物抗肿瘤和抗环磷酰胺致突变作用研究[J].食用菌，2006,28(5):57-59.
• [11]王华林，温万芬.桑黄的药用价值研究进展[J].时珍国医国药，2015,26(11):2747-2750.
• [12]YANG Y,ZHANG J S,LIU Y F,et al.Structural elucidation of a 3-O-methyl-D-galactose-containing neutral polysaccharide from the fruiting bpdies of Phellinus igniarius[J].Carbohydrate Research,2007,342(8):1063-1070.
• [13]窦茜茜，凌沛学，王洪权，等.桑黄子实体两种新多糖的分离纯化和结构研究[J].食品与药品，2009,11(7):21-25.
• [14]PEI J J,WANG Z B,MA H,et al.Structutal featutes and antitumor activity of a novel polysaccharidefrom alkaline extract of Phellinus linteus mycelia[J].Carbohyd Pol,2015(115):472-477.
• [15]高雯雯，张娜，俞淑文.桑黄抗肿瘤及其作用机制的研究进展[J].中国中药杂志，2014,39(21):4165-4168.
• [16]陈晓平，于翠翠.响应面法优化微波辅助乙醇提取桑黄黄酮工艺的研究[J].食品与发酵科技，2013,49(4):31.
• [17]孙佳琦，张甘霖，于明薇，等.桑黄成分及其抗肿瘤作用机制研究进展[J].中国医药导报，2016,13(33):39-42.
• [18]沈业寿，郑立军，王正亮.桑黄胞内多糖抗肿瘤效应的实验研究[J].癌变畸变突变，2007,19(4):305-308.
• [19]IKEDAWA T,NAKANISHI M,UEHARA N,et al.Anei-tumor action of some Basidiomycetes,especially Phellnus linteus[J].Japanese journal of cancer research:Gann,1968,59(2):155-157.
• [20]温克，陈劲，李红，等.桑黄等四种抗癌药物抗癌活性比较[J].吉林大学学报（医学报），2002,28(3):247-249.
• [21]NAKAMURA T,MATSUGO S,UZUKA Y,et al.Fractionation and anti-tumor activity of the mycelia of liquid-cultured Phellinus linteus[J].Biosci Biotechnol Biochem,2004,68(4):868.
• [22]KIM G Y,LEE J Y,LEE J O,et al.Partial character rization and immunostimu latory effect of anovel polysaccharide-protein complex extracted from Phellinus linteus[J].Biosci Biotech Bioch,2006,70(5):1218-1226.
• [23]HAN S B,LEE C W,KANG J S,et al.Acidic polysaccharide from Phellinus linteus inhibits melanoma cell metastasis by blocking cell adhesion and invasion[J].International Immunopharmacology,2006,6(4):697-702.
• [24]VAN GRIENSVEN L J,VERHOEVEN H A.Phellinus linteus polysaccharide extracts increase the mitochondrial membrane potential and cause apoptotic death of THP-1monocytes[J].Chin Med,2013,8(1):25.
• [25]闫景坤，马海乐，祝子坪，等.桑黄菌胞内多糖的理化性质和体外抗氧化活性[J].食品科学，2012,33(9):36-40.
• [26]WANG Z Y,WANG C Y,QUAN Y.Extraction of polysaccharides from Phellinus nigricans mycelia and their antioxidant activities in vitro[J].Carbohydrate Polymers,2014,99:110-115.
• [27]PEI J J,WANG Z B,MA H,et al.Structural features and antitumor activity of a novel polysaccharide from alkaline extract of Phellinus linteus mycelia[J].Carbohyd Pol,2015,115:472-477.
• [28]REN L,PERERA C,HEMAR Y,Antitumor activity of mushroom polysaccharides:a review[J].Food funct,2012,3(11):1118.
• [29]CHEN L,PAN J,LI X,et al.Endo-polysaccharide of Phellinus igniarius exhibited anti-tumor effect through enhancement of cell mediated immunity[J].Int Immunopharmacol,2011,11(2):255-259.
• [30]郭军鹏.缝裂层孔菌抗肿瘤作用及作用机制的研究[D].长春：长春中医药大学，2007.
• [31]杨全，胡旭光，王琦，等.药用真菌桑黄菌丝体多糖抗肿瘤作用的研究[J].中国中药杂志，2006,31(20):1713-1715.
• [32]ZHOU C,JIANG S S,WANG C Y,et al.Different immunology mechanisms of Phellinus igniarius in inhibiting growth of liver cancer and melanoma cells[J].Asian Pac J Cancer Prev,2014,15(8):3659-3665.
• [33]PARK S K,KIM G Y,LIM J Y,et al.Acidic polysaccharides isolated from Phellinus linteus induce phenotypic and functional maturation of murine dendritic cells[J].Biochem Biophys Res Commun,2003,312(2):449-458.
• [34]LEE J J,KWON H K,JUNG I H,et al.Anti-cancer Activities of Ginseng Extract Fermented with Phellinus linteus[J].Mycobiology,2009,37(1):21-27.
• [35]LI X,JIAO L L,ZHANG X,et al.Anti-tumor and immunomodulating activities of proteoglycans from mycelium of Phellinus nigricans and culture medium[J].Int Immunopharmacol,2008,8(6):909-915.
• [36]YANG B K,HWANG S L,YUN I J,et al.Antitumor Effects and Immunomodulating Activities of Phellinus linteus Extract in a CT-26 Cell-Injected Colon Cancer Mouse Model[J].Mycobiology,2009,37(2):128-132.
• [37]HWANG H J,KIM S W.Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190[J].Enzyme Microb Tech,2003,33(2):309-319.
• [38]KIM D H,YANG B K,JEONG S C,et al.Production of a hypoglycemic,extracellular polysaccharide from the submerged culture of the mushroom,Phellinus linteus[J].Biotechnol Lett,2001,23(7):513.
• [39]闫国卿.桑黄和鼠妇化学成分研究[D].合肥：安徽大学，2014.
• [40]莫顺燕，杨永春，石建功.桑黄化学成分研究[J].中国中药杂志，2003,28(4):339-341.
• [41]莫顺燕，杨永春，石建功.桑黄黄酮A和B的分离与合成[J].化学学报，2003(7):1161-1163.
• [42]彭真福，陈庆发，白永亮，等.桑黄残渣黄酮提取工艺研究[J].农产品加工，2014(12):28-35.
• [43]吴长生.药用真菌桑黄化学成分的研究[D].济南：山东大学，2011.
• [44]SHAHIDI F,AMBIGAIPALAN P.Phenolics and polyphenolics in foods,beverages and spices:antioxidant activity and health effects-a review[J].Journal of Functional Foods,2015(18):820-897.
• [45]陈莺莺，陈炳华，赖京菁，等.不同寄主红花寄生叶提取物抗氧化能力的研究[J].福建师范大学学报（自然科学版），2011,27(2):94-100.
• [46]杨文建，李大鹏，李金奎，等.热处理对何首乌抗氧化能力的影响[J].食品与发酵工业，2008,34(11):61-65.
• [47]赵国玲，刘承初，谢晶，等.坛紫菜不同溶剂组分的抗氧化活性[J].食品科学，2010,31(17):186-191.
• [48]LUCRECIA L C,MONICAA N.New method to determine antioxidant activity of polyphenols[J].Food Chemistry,2006(54):8397-8402.
• [49]李有贵，钟石，计东风.野生与人工栽培桑黄子实体中的粗多糖和粗酚含量及药用活性比较[J].蚕业科学，2016,42(5):0883-0891.
• [50]JEONG J B,LEE S H.Protocatechualdehyde possesses anti-cancer activity through downregulating cyclin D1and HDAC2 in human colorectal cancer cells[J].Biochem Bioph Res Co,2013,430(1):381-386.
• [51]LEE J R,LEE M H,EO H J,et al.The contribution of activating transcription factor 3 to apoptosis of human colorectal cancer cells by protocatechualdehyde,a naturally occurring phenolic compound[J].Arch Biochem Biophys,2014,564:203-210.
• [52]CHEN W,ZHAO Z,LI L,et al.Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediate dmitochondrial pathway[J].Free Radical Biology&Medicine,2008,45(1):60-72.
• [53]LU T L,GUAN J H,LU T J,et al.Hispolon from Phellinus linteus has antiproliferative effects via MDM2-recruited ERK1/2 activityin breast and bladder cancer cells[J].Food and Chemical Toxicology,2009,47(8):2013-2021.
• [54]RAVINDTAN J,SUBBARAJU G V,RAMANI M V,et al.Bisdeme-thylcurcumin and structurally related hispolon analogues of curcumin exhibit enhanced prooxidant,anti-proliferative and anti-inflammatory activities in vitro[J].Biochemical Pharmacology,2010,79(11):1658-1666.
• [55]LU T L,HUANG G J,WANG H J,et al.Hispolon promotes MDM2 downregulation through chaperone-mediated autophagy[J].Biochemical and Biophysical Research Communications,2010,398(1):26-31.
• [56]TSAI H H,LEE W R,WANG PH,et al.Propionibacterium acnes-induced iNOS and COX-2 protein expression via ROS-dependent NF-k B and AP-1 activation in macrophages[J].Journal of Dermatological Science,2013,69(2):122-131.
• [57]秦春华，武玲，汪鋆植.9种桑黄子实体中两种酚类活性成分的HPLC含量测定[J].湖北中医药大学报，2015,17(3):36-39.
• [58]黄河胜，马传庚，陈志武.黄酮类化合物药理作用研究进展[J].中国中药杂志，2010,25(10):589.
• [59]黄孝闻，俞忠明，董宇，等.桑黄在古代中医典籍中的应用记载及其现代药用研究进展[J].中华中医药学刊，2014,32(9):2249-2252.
• [60]昝立峰.粗毛纤孔菌与椭圆嗜蓝孢孔菌子实体的化学成分及其药理活性研究[D].长春：吉林农业大学，2012.
• [61]刘凡，庞道睿，邹宇晓，等.桑黄总黄酮含量及其体外抗氧化活性研究[J].中国食用菌，2014,33(2):47-49,56.
• [62]回晶，李其久，边媛媛，等.桑黄总黄酮超声提取工艺及其生物活性研究[J].食品科学，2010,31(24):195-198.
• [63]WANG Y,YU JX,ZHANG CL,et al.Influence of flavonoids from Phellinus igniarius on sturgeon caviar:anrioxidant effects and sensory characteristics[J].Food Chemistry,2012,131(1):206-210.
• [64]钱骅，赵伯涛，陈斌，等.桑黄子实体多糖、黄酮和多酚含量与抗氧化活性相关性[J].食品工业科技，2015,36(12):104-108.
• [65]程鑫颖，包海鹰，丁燕，等.瓦宁木层孔菌中5个化合物的抗肿瘤活性筛选[J].菌物研究，2011,9(3):176-179.
• [66]谢江宁，宋素芬，李香，等.桑黄总三萜的提取及其体外抗脑胶质瘤U251活性[J].中国实验方剂学杂志，2012,18(5):24-26.
• [67]GONXULEZ A G,BARRERA J B,PEREZ M M,et al.Two new triterpene acids from Phellinus pomaceus[J].JChem Soc,Perkin Trans 1,1986,7(32):551.
• [68]GONZALEZ A G,EXPOSITO T S,MARANTE F J T,et al.Lanosterol de-rivatives from phellinus-torulosus[J].Phytochemistry,1994,35(6):1523.
• [69]GONZALEZ A G,SIVERIO E T,BERMEJO B P,et al.The absolute stereochemistry of senexdiolic acid at C-22[J].J Nat Prod,1993,56(12):2170.
• [70]WANG G J,TSAI T H,ChANG T T,et al.Lanostanes from Phellinus igniarius and their iNOS inhibitory activities[J].Planta Med,2009,75(15):1602.
• [71]赵兴华.红缘拟层孔菌子实体的抗肿瘤及抑菌活性的研究[D].长春：吉林农业大学，2010.
• [72]梁佳，孙梦伊，张腾，等.响应曲面法优化桑黄菌丝体中三萜的微波提取工艺[J].中国农学通报，2011,27(10):235.
• [73]于小凤，秦庆玲，李峰，等.响应面法优选桑黄总三萜的超声提取工艺[J].中国药房，2012,23(47):4455-4458.
• [74]昝立峰，包海鹰，李丹花.“桑黄”类真菌中多酚物质及其生物活性研究进展[J].天然产物研究与开发，2016,28:147-155.
• [75]昝立峰，包海鹰，李丹花.“桑黄”类真菌中多酚物质及其生物活性研究进展[J].天然产物研究与开发.2016,28(1):147-155.
• [76]MO S Y,WANG S J,ZHOU G X,et al.Phelligridins C-F:Cytotoxic pyrano[4,3-c][2]benzopyran-1,6-dione and furo[3,2-c]pyran-4-one derivatives from the fungus Phellinus igniarius[J].J Nat Prod,2004,67:823-828.
• [77]MO S Y,YANG Y C,HE W Y,et al.Two pyrone derivatives from fungus Phellinus igniarius[J].Chinese Chem Lett,2003,14:704-706.
• [78]WANG Y,MO S Y,WANG S J,et al.A unique highly oxygenated pyrano[4,3-c][2]benzopyran-1,6-dione derivative with antioxidant and cytotoxic activities from the fungus Phellinus igniarius[J].Org Lett,2005(7):1675-1678.
• [79]WANG Y,SHANG X Y,WANG S J,et al.Structures,biogenesis and biological activities of pyrano[4,3-c]isochromen-4-one derivatives from the fungus Phellinus igniarius[J].J Nat Prod,2007,70(2):296-299.
• [80]LEE I K,HAN M S,LEE M S,et al.Styrylpyrones from the medicinal fungus Phellinus baumii and their antioxidant properties[J].Bioorg Med Chem Lett,2010,20(8):5459-5461.
• [81]MO S Y,YANG Y C,SHI J G,et al.Isolation and synthesis of phelligrins A and B[J].Acta Chim Sin,2003(7):1161-1163.
• [82]莫顺燕，石建功.药用真菌桑黄化学成分研究[D].北京：中国协和医科大学，2003.
• [83]KIN BC,CHOI JW,HONGH Y,et al.Heneocygenase-1mediates the anti-inflammatory effect of mushroom phellinus linteus in LPS-stimulated RA W264.7 macrophages[J].JOURNAL OF Ethnopharmacology,2006,106(3):364-371.
• [84]WU X,LIN S,ZHU C,et al.Homo-and heptanor-sterols and tremulane sesquiterpenes from cultures of Phellinus igniarius[J].J Nat Prod,2010,73(7):1294.
• [85]吴秀丽，林生，朱承根，等.火木层孔菌液体培养物的化学成分研究[J].中国中药杂志，2011,36(7):874.
• [86]涂成荣，张和禹，范涛.桑黄的人工栽培与应用研究进展[J].北方蚕业，2018,39(2):9-13.
• [87]刘红锦，张苏珍，蒋宁，等.桑黄菌丝固体培养基及培养条件研究[J].江西农业学报，2012,24(11):123-124.
• [88]赵昊璐，赵春燕，任蜀豫，等.桑黄固体培养基选择及发菌条件探讨[J].中药材，2008,31(4):482-483.
• [89]骆婷.桑黄的生物学特性及其发酵培养条件的优化[D].合肥：安徽农业大学，2009.
• [90]吕英华.桑黄菌固体和液体培养基优化及人工栽培研究[D].西安：西北农林科技大学，2010.
• [91]许彪，吴佳琳，孙婷婷，等.桑黄人工栽培研究[J].中国林副特产，2015,135(2):91-93.
• [92]杜萍，张春凤，崔宝凯，等.药用真菌桑黄的人工栽培技术研究[J].中国食用菌，2009,28(3):35-37.
• [93]秦俊哲，王雪莲，姚艳芳.桑黄菌子实体分化发育条件初探[J].食用菌，2008,30(1):31-32.
• [94]郭照辉，刘红.桑黄菌的特性及人工栽培技术[J].南方园艺，2013,24(4):54-56.
• [95]杨宏伟，丁伟，杨永顺.桑黄人工栽培技术及经济效益分析[J].黑龙江农业科学，2006(4):70-71.
• [96]储锡霞，崔月花，王勇富.桑黄液体深层发酵培养基优化的研究[J].食用菌，2010(4):14-16.
• [97]雷萍，张文隽，吴亚召，等.桑树桑黄液体发酵培养基优化初报[J].食用菌，2010(6):15-16.
• [98]傅海庆，周阳，傅华英，等.药用真菌桑黄的液体发酵培养基的配方优化筛选研究[J].江西农业大学学报，2012,34(5):1039-1042.
• [99]赵子高，杨焱，刘艳芳，等.药用真菌桑黄液体深层发酵条件的优化[J].微生物学通报，2007,34(3):459-462.
• [100]祝明思，王应男，张公亮，等.桑黄菌丝体液体发酵培养条件的研究[J].中国酿造，2010,224(11):88-91.
• [101]姚竞.药用真菌桑黄袋栽驯化条件试验[J].食药用菌，2018,26(6):381-382,390.
• [102]王勇.陕南地区桑黄菌室内袋料栽培技术[J].特种经济动植物，2019(9):34-36.
• [103]刘艳，黄传书，赵珮，等.桑树桑黄人工繁育技术研究[J].蚕学通讯，2019,39(2):5-9.
• [104]周蔓，李萍，何俊蓉.不同培养条件对桑黄菌丝体生长的影响[J].现代中药研究与实践，2017,31(2):1-8.
• [105]施阳阳，蒲彬，刘燕林，等.桑枝屑不同添加量对桑黄的生物转化率和质量的影响[J].蚕学通讯，2019,39(2):1-4.
• [106]吴声华，戴玉成，杨宏.桑黄真菌分类学研究[C]//中国菌物学会、台湾真菌学会、中国科学微生物研究所.海峡两岸第十届菌物学暨第三届食药用菌学术研讨论文摘要集.杭州：[出版者不详]，2011:2.
• [107]ZHOU L W,VLASAK J,DECOCK C,et al.Global diversity and taxonomy of the Inonotus linteus complex(Hymenochaetales Basidiomycota):Sanghuangporus Gen.nov.Tropicoporus excen-trodendri and T.guanacastensis gen.et spp.Nov.and 17 new combinations[J].Fungal Diversity,2016,77(1):335-347.

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